Table 1.
Primers used for gene expression analysis by RT-PCR
| Cycle number(N) | ||||||
| Exp1 | Exp2 | |||||
| Gene product | Primer Sequence | bp | Ta (°C) | Liv | Tes | Liv |
| Estrogen receptor α (ERα, AJ006039) | 5'-CCCATCCAGTCAGCATTCA-3' 5'-TTGTCACGCCGCAGAACG-3' |
374 | 57 | 25 | 25 | 25 |
| Estrogen receptor βa (ERβa, AF136980) | 5'-GCTGATGATCGGACTGATGTG-3' 5'-GGTGTACTGTTGGCGGAAAG-3' |
348 | 59 | 27 | 23 | 29 |
| Estrogen receptor βb (ERβb, AJ580049) | 5'-TGATGATGTCACTCACCAACC-3' 5'-TTCAGCTCACGAAACCGA-3' |
291 | 54 | 25 | 22 | 26 |
| Choriogenin L (ChgL, CX734876) | 5'-AGAGGGATGCTGTCGTAG-3' 5'-GTGATGCCTTTGGTAGTG-3' |
290 | 56 | 25 | 30 | 18 |
| Vitellogenin II (VgII, CX734956) | 5'-CACTTGGCATTGGTCTCCC-3' 5'-ATGGTGCACTCAGCTGCATG-3' |
130 | 58 | 20 | 25 | 18 |
| 18S ribosomal RNA (18S) | 5'-TCAAGAACGAAAGTCGGAGG-3' 5'-GGACATCTAAGGGCATCACA-3' |
495 | 59 | 18 | 18 | 15 |
Gene name abbreviation and accession number, primer sequence (forward and reverse, respectively), amplicon length (bp), annealing temperature (Ta) and optimized cycle numbers (N) for liver (Liv) or testis (Tes) cDNAs from Experiments (Exp) 1 and 2.