Figure 5.

Expression of fibronectin and laminin within neurospheres. Frozen sections of mouse neurospheres were prepared as described in Methods and expression of fibronectin and laminin examined by immunofluorsecent microscopy. Neurospheres were labelled with normal rabbit serum (B), or primary antibodies against fibronectin (D) or laminin (F), before labelling with a Cy-3 conjugated secondary anti-rabbit antibody. All cell nuclei of the neurospheres were identified by Hoechst labelling (A, C, E). Scale bar = 100 μm. Note that fibronectin was expressed throughout the neurosphere, with a pattern that outlined individual cell bodies, while laminin labelled cell processes between cells, and was expressed predominantly by cells nearer the centre of the neurosphere.