Figure 6.

Analysis of the roles of specific integrins in mediating brain EC attachment to neurospheres. Cell-tracker-labelled mouse brain EC were plated onto mouse neurospheres, in the presence of the different integrin function-blocking reagents: RGD peptides, the β1 integrin monoclonal antibody Ha2/5, or the α6 integrin monoclonal antibody GoH3. Control DGR peptides, or an isotype-matched monoclonal antibody were included for controls. 18 hours later, EC adhesion to neurospheres was quantified. Adhesion is expressed as the percentage of EC adhesion to neurospheres under control conditions (no reagents added); all points represent the mean ± SD of four experiments. * indicates statistically significant effect (p < 0.01). Note that EC adhesion to neurospheres was inhibited by RGD peptides and the β1 integrin monoclonal antibody, but not by the α6 integrin antibody.