Figure 3.

Viability of cultured cells of Spodoptera frugiperda (Sf-21) expressing the murine sEH (MsEH) in the absence (●) or presence (▴) of compound 3 at 60 μM. Cells expressing β-galactosidase (Lac Z; ♦) were used as control. (a) Effect of trans-stilbene oxide from 0 to 1 mM. sEH detoxifies trans-stilbene oxide (Inset) to its diol, and the cytotoxicity of stilbene oxide is enhanced by the sEH inhibitor DCU. (b) Effect of leukotoxin/isoleukotoxin methyl ester from 0 to 1 mM. sEH activates leukotoxin to its toxic diol, and the cytotoxicity of this compound is reduced dramatically by the sEH inhibitor DCU. Results are means of three separate experiments; bars = SD. Experiments were performed as described previously (32).