Figure 4.

Detection of neutrophil defensins in epithelial cells isolated from mucosa with active ulcerative colitis. Acetic acid extracts of ulcerative colitis (UC) and normal small intestinal (SI) epithelial cells were subjected to acid urea–polyacrylamide gel electrophoresis and transferred to a PVDF membrane. Synthetic human neutrophil defensin peptide 1 (HNP-1; 2.5 μg) was also run as shown. (A) Amido black stain for protein. (B) Duplicate lanes probed with anti-HNP 1–3 antibody; a band immunoreactive for HNP is clearly seen in the lane with the UC epithelial cell extract (arrowed), but not in the lane containing the normal small intestinal epithelial cell extract.