Figure 2.
Expression of MDR related proteins in normal human liver tissue. (A) MDR1 P-gp, MDR3 P-gp, MRP2, and BCRP show similar staining at the canalicular membranes of the hepatocytes (example of staining with M2I-4). (B) MRP3 is present in the bile ducts and the basolateral membranes of the hepatocytes (staining with M3II-9). (C) negative control. Slides were stained with HRP labelled rabbit antimouse, FITC labelled tyramine, HRP labelled rabbit anti-FITC, and aminoethylcarbazole. BCRP, breast cancer resistance protein; FITC, fluorescein isothiocyanate; HRP, horseradish peroxidase; MDR, multidrug resistance.