Table 2.
Detection of MDR molecules with a panel of monoclonal antibodies in formalin fixed, paraffin wax embedded normal human tissues and in frozen sections of different species
| MDR molecule | Mab | Paraffin wax embedded sections | Frozen sections | |||
| Human | Human | Rat | Mouse | Guinea pig | ||
| MDR1 P-gp | JSB-1 | + | ++ | − | − | − |
| C219 | ++ | +++ | ++ | + | ++ | |
| MDR3 P-gp | P3II-1 | +/− | ++ | +/− | − | +/− |
| P3II-26 | +/− | +++ | + | +/− | ++ | |
| MRP1 | MRPr1 | + | +++ | − | ++ | ++ |
| MRPm6 | + | + | − | − | − | |
| MRPm5 | +/− | + | − | − | − | |
| MRP2 | M2I-4 | + | +++ | − | − | − |
| M2II-12 | − | ++ | − | − | − | |
| M2III-5 | − | ++ | +++ | − | + | |
| M2III-6 | + | ++ | +++ | − | ++ | |
| MRP3 | M3II-9 | − | ++ | − | − | − |
| M3II-21 | − | ++ | − | − | − | |
| BCRP | BXP-21 | + | + | +/− | − | − |
| BXP-34 | − | + | +/− | − | − | |
| MVP | LRP-56 | + | ++ | − | − | − |
| LMR-5 | + | + | − | − | − | |
| MVP-37 | ++ | ++ | − | − | − |
Reactivity of MDR1 P-gp, MDR3 P-gp, MRP2, MRP3, and BCRP as judged on sections of liver, and reactivity of anti-MRP1 and MVP Mabs as judged on sections of lung. Staining scores: −, no reactivity; +/−, very weak reactivity; +, weak reactivity; ++, strong reactivity; +++, very strong reactivity.
BCRP, breast cancer resistance protein; Mab, monoclonal antibody; MDR, multidrug resistance; MRP, multidrug resistance protein; MVP, major vault protein; P-gp, P-glycoprotein.