Table 1.
IgA tTG commercial enzyme linked immunosorbent assay kits: manufacturers' kit details
| Manufacturer | Source of tTG | Calcium activated tTG | Serum dilution | Incubation times (serum, conjugate) in minutes | Conjugate | Substrate |
| AESKULISA/Aesku.Lab Diagnostica (Wendelsheim, Germany) | Recombinant human | NS | 1/100 | 30, 15 | HRP antihuman IgA | TMB |
| The Binding Site (Birmingham, UK) | Guinea pig liver | Yes | 1/100 | 30, 30 | Rabbit HRP antihuman IgA | TMB |
| Recombinant human | No | 1/100 | 30, 30 | Rabbit HRP antihuman IgA | TMB | |
| Eurospital S.p.A (Trieste, Italy) | Guinea pig liver | NS | 1/25 | 60, 60 | Sheep HRP antihuman IgA | TMB |
| Recombinant human | NS | 1/25 | 60, 60 | Sheep HRP antihuman IgA | TMB | |
| Genesis Diagnostics (Littleport, UK) | Guinea pig liver | Yes | 1/100 | 30, 30 | Rabbit HRP antihuman IgA | TMB |
| ImmuLisa/Immco Diagnostics Inc (Buffalo, New York, USA) | Guinea pig liver | NS | 1/50 | 60, 30 | Alkaline phosphatase antihuman IgA | PNPP |
| Immunopharmacology Research Diagnostics (Catania, Italy) | Guinea pig liver | NS | 1/100 | 30, 30 | HRP antihuman IgA† | TMB |
| QUANTA Lite/Inova (Diagnostics Inc, San Diego, California, USA) | Guinea pig liver | NS | 1/100 | 30, 30 | Goat HRP antihuman IgA | TMB |
| Purified human erythrocyte | NS | 1/100 | 30, 30 | Goat HRP antihuman IgA | TMB | |
| Medizyme/Medipan Diagnostica GmbH (Selchow, Germany) | Guinea pig liver | NS | 1/50 | 60, 30 | Sheep HRP antihuman IgA | TMB |
| Orgentec Diagnostika GmbH (Mainz, Germany) | Purified human | NS | 1/100 | 30,15 | Rabbit HRP antihuman IgA | TMB |
| Varelisa/Pharmacia & Upjohn Diagnostics GmbH & Co (Freiburg, Germany) | Recombinant human | NS | 1/100 | 30, 30 | HRP antihuman IgA | TMB |
†In the manufacturer's kit insert, alkaline phosphatase was mentioned under “Principle”, but HRP was mentioned under “Reagent supplied”.
HRP, horseradish peroxidase; NS, not stated; PNPP, paranitrophenyl phosphate; TMB, 3,3`,5,5` tetramethylbenzidine; tTG, tissue transglutaminase.