Figure 3.

Comparison of frequency of SCF-directed infection of M-O7e cells with infection of K562 cells with AdGFP. MO-7e and K562 cells were either untreated, treated with 100 μl of bio-AdGFP (moi = 100), or treated with 100 μl of the SCF-directed vector constructed with 250 ng/ml bio-SCF (moi = 100). Twenty-four hours later, K562 cells uninfected (a) and infected with bio-AdGFP (b) and MO-7e cells uninfected (e), infected with bio-AdGFP (e), and infected with SCF-targeted bio-AdGFP (f) were analyzed by FACS for GFP expression. K562 cells infected with AdGFP (c and d) and MO-7e cells infected with SCF-targeted bio-AdGFP (g and h) also were analyzed by photomicroscopy for 4′,6-diamidino-2-phenylindole staining and expression of GFP. Photographs were taken at ×400. Results are representative of two experiments.