Table 1.

Antibody characteristics

Antibody/antigen	Type	Source	Dilution	Incubation	Pretreatment	Cell pattern
Calretinin	P	Zymed (San Francisco, USA)	1/50	Overnight/4° C	Hotplate	Cytoplasm
CEA	M	Dako (Ely, UK)	1/20	30–60 min/RT	Microwave	Cytoplasm
Cytokeratin 5/6	M	Dako	1/50	30–60 min/RT	Microwave	Pericellular and cytoplasm
BerEP4	M	Dako	1/50	30–60 min/RT	Pronase	Membrane
E-cadherin	M	Zymed	1/25	Overnight/4° C	Hotplate	Membrane
HBME-1	M	Dako	1/100	30–60 min/RT	No pretreatment	Membrane and cytoplasm
LeuMI (CD15)	M	Dako	1/40	Overnight/4° C	Microwave	Cytoplasm
N-cadherin	M	Zymed	1/25	Overnight/4° C	Hotplate	Cytoplasm
SP-A	P	Chemicon (Harrow, UK)	1/50	Overnight/4° C	Hotplate	Membrane and cytoplasm
Thrombomodulin	M	Dako	1/100	Overnight/4° C	No pretreatment	Membrane
TTF-1	M	Lab Vision (Newmarket, UK)	1/50	30–60 min/RT	Microwave	Nucleus

CEA, carcinoembryonic antigen; M, monoclonal mouse; P, polyclonal rabbit; RT, room temperature; SP-A, surfactant apoprotein A; TTF-1, thyroid transcription factor 1.