Figure 1.

Image capture and calculation of the adipocytic ratio; all images von Kossa, original magnification, ×40; (A) Live image of representative field of bone marrow captured digitally; (B) adipocytic tissue (darker areas) highlighted by semi-automated selection of area above grey scale cutoff; (C) area for calculation of adipocytic ratio (large dark area) selected with cursor, omitting cancellous struts and artefacts; (d) adipocytic tissue (darker areas) highlighted by subtraction from grey/haemopoietic tissue within the area selected and the ratio of clear/adipocytic to grey/haemopoietic stromal tissue calculated.