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Expression of α smooth muscle actin (ASMA) and CD34 in diffuse-type advanced gastric carcinoma tissue. Stains for (A) ASMA, (B) high molecular weight caldesmon (HCD), and (C) low molecular weight cytokeratins (CK). ASMA positive stromal cells are detected in the tumour stroma. Immunoreactivity for HCD is shown to distinguish ASMA positive stromal cells from smooth muscle cells. Immunoreactivity for CK is shown to identify diffuse-type gastric carcinoma cells.
