Figure 6.

Lack of detection of hGAA mRNA in quadriceps and heart tissues of AdhGAAΔpol-treated GAA-KO mice. Respective tissues from a C57BL/6 mouse or tissues derived from GAA-KO mouse that had been given AdhGAAΔpol 12 days prior were harvested, and total RNA was analyzed for the presence of hGAA, as described in Materials and Methods. The Ad-treated mouse had GAA activities (nmol/hr/mg) of 44.3 in the heart, 214.7 in the quadriceps muscle, and 21,790 in the liver whereas C57BL/6 mice had average GAA activities of 22.15 in the heart, 10.2 in the quadriceps, and 98.7 in the liver. The amount of 28s rRNA present in each sample before probing is presented as well.