Periodic acid-Schiff detection of glycogen in situ. Cryosections from 2.5-month-old mouse tissues were periodic acid-Schiff stained for glycogen as described in Materials and Methods. Source of the tissue sections are as follows: (a) GAA-KO, gastrocnemius, untreated. (b) GAA-KO, gastrocnemius, 12 days post-AdhGAAΔpol. (c) GAA-KO, quadriceps, untreated. (d) GAA-KO, quadriceps, 12 days post-AdhGAAΔpol. (e) GAA-KO, diaphragm, untreated. (f) GAA-KO, diaphragm, 12 days post-AdhGAAΔpol. (g) GAA-KO, heart, untreated. (h) GAA-KO, heart, 4 days post-AdhGAAΔpol. (i) GAA-KO, heart, 12 days post-AdhGAAΔpol. (j) Wild-type, heart, untreated.