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Results of a typical multiplex reverse transcription polymerase chain reaction for human defensin 5 (HD-5), HD-6, human β defensin 1 (HBD-1), and HBD-2, with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal standard. Lane 1, oesophagus; lane 2, fundus; lane 3, corpus; lane 4, antrum; lane 5, duodenum; MW, molecular weight marker. Biopsies were obtained from two patients with Helicobacter pylori (Hp) negative and H pylori positive chronic gastritis.
