Table 1.

Sequences of primers used

Primers	Region	Nucleotide sequence
CMV
Outer PCR
CMVR-1	IE exon 4	GTAATGAAGCGCCGCATTGAGGAGA
CMVL-1	IE exon 4	GGCAACTTCCTCTATCTCAGACAC
Inner PCR
CMVR-2	IE exon 4	CCTAGTGTGGATGACCTACGGGCCA
CMVL-2	IE exon 4	CAGACACAGTGTCCTCCCGCTCCTC
Chlamydia pneumoniae
HL-1		GTTGTTCATGAAGGCCTACT
HR-1		TGCATAACCTACGGTGTGTT
β actin
β3		TGGACTTCGAGCAAGAGATG
β4		CTCCTTCTGCATCCTGTC

Primers for CMV are based on the sequence published by Kouzarades et al,²⁴ and for C pneumoniae on that by Campbell et al.²⁵

CMV, cytomegalovirus; PCR, polymerase chain reaction.