Figure 4.

Determination of the cleavage sites produced by the Hjc. An immobile four-way junction (A and B) and a mobile junction (C and D), each labeled uniquely at the 5′-³²P-end in indicated (∗) strand, were used as substrates. Substrate DNAs were incubated with (+) or without (−) Hjc protein. The GA sequence ladders of each labeled oligonucleotide generated by the Maxam–Gilbert method were loaded alongside to provide markers. The cleavage sites are represented by arrowheads showing cutting efficiencies by their sizes. The box part of the junctions in D indicates the mobile homologous region.