Study of viral integration. For the detection of integration, the E2 open reading frame of HPV-16 was amplified. Examples of viral genomic integration (lanes 1, 5, 6, 9, 11, and 13) and of viral episomal forms (lanes 10, 12, 14, 16, 17, 20, and 21) are shown. Arrows on the right show the expected molecular weight of the E2 gene fragment (351 bp).