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. 2003 Oct;56(10):728–730. doi: 10.1136/jcp.56.10.728

Figure 1.

Figure 1

Real time polymerase chain reaction protocol 1 using serial 10 fold dilutions of control SV40 DNA from 10−4 pg down to 10−8 pg. Amplification occurred at 10−4 pg at 29 cycles, for 10−5 pg at 32 cycles, and for 10−6 pg (approximately one virus copy) at 34 cycles. There was no amplification for 10−7 or 10−8 pg.