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. 1999 Aug 3;96(16):8879–8883. doi: 10.1073/pnas.96.16.8879

Figure 1.

Figure 1

(A) Fibrin-independent Pg activation by different rSKs and Glu-Pg as a function of time. The various rSKs (10 nM) were added to a quartz cuvette containing human Glu-Pg (300 nM) and S2251 (see Table 1) in 50 mM Tris⋅HCl/100 mM NaCl, pH 7.4 at 37°C. The rate of substrate cleavage was monitored by continuously recording the change in absorption at 405 nm. (B) Restoration of fibrin-independent Pg activation to rSKΔ59 by rSK1–59. rSKΔ59 (10 nM), rSK1–59 (10 nM), rSK1–59 (10 nM) + rSKΔ59 (10 nM), or no SK was mixed with Glu-Pg (300 nM) and S2251 substrate (0.5 mM), and the rate of substrate cleavage was monitored as above.