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pRb immunostaining of three breast carcinomas using two different monoclonal antibodies: (A, B) clone G3-245, which specifically recognises the phosphorylated pRb form, and (C) clone 1F8/Rb1, which reacts with total pRb. (A) Only a few cancer cells are positive in this case but (B) a high number of nuclei are positive in this case. (C) The cancer cells are unstained, indicating a presumable deletion of the pRb gene.
