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Selecting antibodies from phage libraries. (1) Phage are incubated with the antigen of interest—any phage that express a single chain heavy and light chain variable region fragment (scFv) that binds to the antigen. (2) Phage that express scFv fragments that do not bind to the antigen are washed away. (3) Phage that express scFv fragments that bind to the antigen are isolated, expanded, purified, and then used in further applications.
