Table 2.

 Oligonucleotide primers used to amplify the 16S rRNA and Helicobacter pylori specific genes

Gene	Sequence (5′ to 3′)	Reaction conditions	Amplicon size (bp)	Position
16S rRNA	GTCATGACGGGTATCC ACTTCACCCCAGTCGCTG	94°C for 1 min, 55°C for 2 min, 72°C for 2.5 min (35 cycles)	1200 bp	276–291 1495–1478
		98°C for 10 min (1 cycle)
26 kDa	TGGCGTGTCTATTGACAGCGAGC	94°C for 30 sec, 68°C for 1 min (37 cycles)	298 bp	474–496
	CCTGCTGGGCATACTTCACCATG	94°C for 30 sec, 68°C for 1 min, 72°C for 2 min (6 cycles)		776–754
cagA	ATAATGCTAAATTAGACAACTTGAGCGA TTAGAATAATCAACAAACATCACGCCAT	94°C for 1 min, 55°C for 1 min, 72°C for 1.5 min (35 cycles)	297 bp	1751–1778 2048–2021
glmM	GGATAAGCTTTTAGGGGTGTTAGGGG GCTTACTTTCTAACACTAACGCGC	94°C for 1 min, 56°C for 1 min, 72°C for 1 min (35 cycles)	296 bp	1289–1314 1584–1561
vacA	GGAGCCCCAGGAAACATTG CATAACTAGCGCCTTGCAC	94°C for 1 min, 52°C for 1 min, 72°C for 1 min (35 cycles)	352 bp	976–994 1327–1309
rps4	ATGAGTCTTTCTTCATATGGGA ATGGCAAGATATAGAGGTGCAG	94°C for 1 min, 58°C for 1 min, 72°C for 1 min (40 cycles)	605 bp	605–584 1–22