Figure 5.
(A) Ethidium bromide stained 10% polyacrylamide gel of polymerase chain reaction products (VH and JH primers) from DNA samples as follows: MW, molecular weight markers (size in base pairs); P, positive control; N, negative control; R, reagent control (no DNA); lanes 1 and 2, two distinct clonal bands from the same paraffin wax block of Hashimoto’s thyroiditis from two sections 25 μm apart. (B) The clonal bands in lanes 1 and 2 have different rearranged VDJ region sequences of the immunoglobulin heavy chain gene (IgH) (top from lane 1 and bottom from lane 2).