Table 2.

 Comparison of immunohistochemistry and western blot results in the index and control placentas

Method	Index placenta	Control placentas
Immunohistochemistry	Slight and discontinuous AP staining in syncytiotrophoblast brush border membrane	Intensive continuous AP staining in syncytiotrophoblast brush-border membrane
	8–10% Ki-67 positive syncytiotrophoblast cells/field on average	1–2% Ki-67 positive syncytiotrophoblast cells/field on average
Western blot	Total-Akt 103%	Total-Akt 100%
	Total-GSK-3β 106%	Total-GSK-3β 100%
	Phospho-Akt 174%	Phospho-Akt 100%
	Phospho-GSK-3β 152%	Phospho-GSK-3β 100%
	Phospho-Erk 1/2 561%	Phospho-Erk 1/2 100%
	Phospho-SAPK/JNK 202%	Phospho-SAPK/JNK 100%
	p38MAPK 249%	p38MAPK 100%

Akt, protein kinase B; AP, alkaline phosphatase; GSK-3β, glycogen synthase kinase-3β; p38-MAPK, p38 mitogen activated protein kinase; Erk1/2, extracellular signal regulated kinase1/2; SAPK/JNK, stress activated protein kinase/c-Jun N-terminal kinase.