Table 1.

 Immunoreactivity of markers showing discrepant staining according to the fixative used

Antigen	Location	Bouin’s		Formalin
		Number of slides (cases)	Results	Number of slides (cases)	Results
MCM2	Nuclear	6 (5)	Negative	22 (11)	Good
p27	Nuclear	109 (23)	Decreased	10 (5)	Good
C-erb-B2	Membrane	23 (4)	Aberrant	15 (7)	Good

Good: the staining pattern was consistent with the expectation based on previous reports; decreased: for p27, a lower percentage of staining was seen, with persistent cytoplasmic staining; aberrant: for C-erb-B2, an altered pattern of spatial expression was noted—although the staining was crisp, the location of the staining was aberrant.