Table 1.

 Primer sequences used to amplify BRCA1 exon by exon for denaturing gradient gel electrophoresis¹²

Fragment	Forward primer	Reverse primer	Size (bp)
Exon 2-1	ATGATAAAATGAAGTTGTC*	ACACTCTAAGATTTTCTGC	204
Exon 2-2	TTATCTGCTCTTCGCGTTG	CTTCCCTAGTATGTAAGGTC*	202
Exon 3	GCGCGTTGAGCCTCATTTATTTTC	ACAAAAGCTAATAATGGAGC*	185
Exon 5	GTATTCTTTCTACAAAAGG*	TCCAACCTAGCATGATTAC	208
Exon 6	GGTTGATAATCACTTGCTG*	CACTTGAGTTGCATTCTTG	223
Exon 7	ATACATAGGGTTTCTCTTG*	AGAAGAAAACAAATGGTTT	293
Exon 8	TTGCTTGACTGTTCTTTACC*	ACTTAAAAAACCTGAGACC	209
Exon 9	CCCTTTTAATTAAGAAAAC*	ACTAAATAGGAAAATACCAG	191
Exon 10	CATTTGACAGTTCTGCATAC*	TTCAGTGCCTGTTAAGTTG	217
Exon 11-1	ATGACAATTCAGTTTTTGAG*	TATTACTGGGTTGATGATG	147
Exon 11-2	AGCTGCTTGTGAATTTTCTG*	ATAAACTGCTGTTCTCATGC	243
Exon 11-3	TTTTTACAAATACTCATGCCAGCTC*	TAGGATTCTCTGAGCATGGC	313
Exon 11-4	TTTTTTTTTTTGTGTGAGAGAAAAGAATGG*	CATCTACCTCATTTAGAACG	272
Exon 11-5	GAATCAAATGCCAAAGTAGC*	GGACGCTCTTGTATTATCTG	329
Exon 11-6	ATTATAGGAGCATTTGTTAC	TTTTCGAGTGATTCTATTGG*	324
Exon 11-7	CAAAAGGTGATTCTATTCAG*	ATAAGGTGGGCTTAGATTTC	275
Exon 11-8	CAGGCATATTCATGCGCTTG*	GAAAGTATCGCTGTCATGTC	281
Exon 11-9	GAGTAACAAGCCAAATGAAC	GGGGTCTTCAGCATTATTAG*	242
Exon 11-10	TTGTCAATCCTAGCCTTCC*	ATTAGTCCCTTGGGGTTTTC	324
Exon 11-11	AATAAATGTGTGAGTCAGTG*	ACATTCCTCTTCTGCATTTC	300
Exon 11-12	ATTCAAGGTTTCAAAGCGCC	GTATATTAACTGTCTGTAC*	238
Exon 11-13a	AGGAAGAAAATCAAGGAAAG*	TAATGAGTCCAGTTTCGTTG	223
Exon 11-13b	GCCAAATGTAGTATCAAAGG	CAGGTGACATTGAATGTTCC*	248
Exon 11-13c	AAAATCTGCTAGAGGAAAAC	TCATCACTGGAACCTATTTC*	259
Exon 11-13d	TAAAGAAGCCAGCTCAAGC	CTGAAATCAGATATGGAGAG*	325
Exon 11-13e	GCAAGAATGTCAAGAAGTAG*	CCATCATCTAACAGGTCATC	181
Exon 11-13f	AGTCATGCATCTCAGGTTTG*	ATAAGTTCTCTTCTGAGGAC	280
Exon 11-14	CTTTCACCCATACACATTTG	TGCAGTCATTTAAGCTATTC*	277
Exon 11-15	GAGTGTCTGTCTAAGAACAC*	TATTTGCAGTCAAGTCTTCC	221
Exon 11-16	GTTTCTTCACAGTGCAGTG	AAATAGACTGGGGCAAACAC*	296
Exon 12	GTCTGCTTTTACATCTGAACC*	AATGCAAAGGACACCACACAC	221
Exon 13	GCGCGATTTCATTTTCTTGGTGCC	GGGAAGGAAAGAATTTTGC*	305
Exon 14	TCAGAACAAAGCAGTAAAG*	AAGATGTCAGATACCACAG	257
Exon 15-1	ATTGGTGGCGATGGTTTTC*	CTCCTCCACATCAACAACCT	204
Exon 15-2	ACTACCCATCTCAAGAGGAG	AAATCAAAGTGTTTGTTCC*	195
Exon 16-1	GACCAGAACTTTGTAATTC*	CCAGCAGTATCAGTAGTAT	299
Exon 16-2	AAAGTTGCAGAATCTGCCC	TAAGTCTTAGTCATTAGGG*	252
Exon 17	GTGCTAGAGGTAACTCATG*	CAGCAGATGCAAGGTATTC	213
Exon 18	ACAGCACTTCCTGATTTG	TCTGAGGTGTTAAAGGGAG*	222
Exon 19	TCTATCTCCGTGAAAAGAG	CTGGTTAGTTTGTAACATC*	176
Exon 20	TGCTCCACTTCCATTGAAG*	TTTGTCAACTTGAGGGAGG	220
Exon 21	CCTCTCTCCATTCCCCTG*	AAGGCTGGTGCTGGAACTC	182
Exon 22	GCCTGGGTTAAGTATGCAG*	ATTGTGTCCTCCCTCTCTG	210

*GC clamp sequence (CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCG) is attached to this site.