Table 3.

 Amplicon pools created for BRCA1 to increase the throughput of denaturing gradient gel electrophoresis

Gene	Pool	Exons in amplicon pool
BRCA1	A	7,18, 10, 20
	B	9, 6, 8, 23
	C	5, 2B, 11-3, 16B
	D	14, 11-4, 22, 24
	E	2A, 11-1, 16A
	F	19, 13, 21
	G	17, 11-14, 3
	H	11-15, 15A, 15B
	I	11-16, 11-2, 12
BRCA1 exon 11	A	11-5, 11-8, 11-10
	B	11-13b, 11-13e, 11-13f
	C	11-7, 11-12, 11-13c
	D	11-9, 11-11, 11-13a
	E	11-6, 11-13d

Each amplicon pool contained three to five amplicons with relatively large melting temperature differences.