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. 2005 May;58(5):509–514. doi: 10.1136/jcp.2004.020347

Table 2.

 Primer and probe sequences used in real time quantitative polymerase chain reaction

Gene Primer sequence (5′–3′) Hybridisation probe sequence (5′–3′)
CCNA
    Sense AAGAAGCCAGCTGAATCTCAAA AAAAGCCAGGGCATCTTCACGCTCTATT
    Antisense GGTCCAGGTAAACTAATGGCTGAA
CCNB1
    Sense CCCTGCTGCAACCTCCAA CCCGGACTGAGGCCAAGAACAGC
    Antisense TGTTCACTGACTTTGTTACCAATGTC
CCNC
    Sense GCCGGCTGGTGCTTTTTTA TGCCATGGAACACAGCTTGCCCT
    Antisense TGGGAGCTCTGCCAAAAGTT
CCND1
    Sense CCGTCCATGCGGAAGATC CCTCCAGCATCCAGGTGGCGA
    Antisense AACAAGTTGCAGGGAAGTCTTAAGA
CCND3
    Sense CTGTCTCTCCCCGCCAGTT CACCCCCGACACGTATTGTCT CCC
    Antisense CTGATATCTCAAGCTTTCCTTTTCCT
CCNE
    Sense CCCCGCTGCCTGTACTGA TCAGTGCCGACTCTGCCACATGG
    Antisense AGCATGGAGTAAGAGACCTGGAA
CCNH
    Sense TATCCTTACCACACTTTTTTTCCTTCT TCTACCAGGTCGTCATCAGTCCATTCTTCCT
    Antisense AGAAATCAACTTCAAATGGTTAGAGAGA
HSA
    Sense TGTTGCATGAGAAAACGCCA AAGTGACAGAGTCACCAAAT GCTGCACAG
    Antisense GTCGCCTGTTCACCAAGGAT

All probes were labelled at the 5′ end with FAM as reporter and at the 3′ end with TAMRA as quencher.

HSA, human serum albumin.