Table 2.
Gene | Primer sequence (5′–3′) | Hybridisation probe sequence (5′–3′) |
CCNA | ||
Sense | AAGAAGCCAGCTGAATCTCAAA | AAAAGCCAGGGCATCTTCACGCTCTATT |
Antisense | GGTCCAGGTAAACTAATGGCTGAA | |
CCNB1 | ||
Sense | CCCTGCTGCAACCTCCAA | CCCGGACTGAGGCCAAGAACAGC |
Antisense | TGTTCACTGACTTTGTTACCAATGTC | |
CCNC | ||
Sense | GCCGGCTGGTGCTTTTTTA | TGCCATGGAACACAGCTTGCCCT |
Antisense | TGGGAGCTCTGCCAAAAGTT | |
CCND1 | ||
Sense | CCGTCCATGCGGAAGATC | CCTCCAGCATCCAGGTGGCGA |
Antisense | AACAAGTTGCAGGGAAGTCTTAAGA | |
CCND3 | ||
Sense | CTGTCTCTCCCCGCCAGTT | CACCCCCGACACGTATTGTCT CCC |
Antisense | CTGATATCTCAAGCTTTCCTTTTCCT | |
CCNE | ||
Sense | CCCCGCTGCCTGTACTGA | TCAGTGCCGACTCTGCCACATGG |
Antisense | AGCATGGAGTAAGAGACCTGGAA | |
CCNH | ||
Sense | TATCCTTACCACACTTTTTTTCCTTCT | TCTACCAGGTCGTCATCAGTCCATTCTTCCT |
Antisense | AGAAATCAACTTCAAATGGTTAGAGAGA | |
HSA | ||
Sense | TGTTGCATGAGAAAACGCCA | AAGTGACAGAGTCACCAAAT GCTGCACAG |
Antisense | GTCGCCTGTTCACCAAGGAT |
All probes were labelled at the 5′ end with FAM as reporter and at the 3′ end with TAMRA as quencher.
HSA, human serum albumin.