Table 4.

 Amplification of cyclin genes in primary human colon adenocarcinoma

Gene	N (total)	Amplification level*
		<0.5	0.5–1.9	2.0–4.9	⩾5
CCNA	210	1	196	13	0
CCNB1	188	2	169	16	1
CCNC	208	1	151	39	17
CCND1	209	0	94	96	19
CCND3	204	6	155	39	3
CCNE	204	1	164	36	3
CCNH	197	8	179	10	0

*N-fold difference from the normal control. Cutoff level, >2. Amplification was measured by real time quantitative polymerase chain reaction and levels were determined by the comparative C_t method (2^−ΔΔCt method).