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Sensitivity of pooling DNA samples. A heterozygous single nucleotide polymorphism was identified in MYH7 intron 38 (23485 G>A) in a sample. Various amounts of known wild-type sample (10 ng/sample) were mixed with the above heterozygous sample before polymerase chain reaction amplification. Different profiles after normalisation by Navigator software are shown (one variant in 2–40 copies of normal alleles). The homozygous wild-type control is indicated by a dashed line.
