Northern blot analysis of antimicrobial peptide expression in the gastrointestinal tract. RNA from adult human non-diseased gastrointestinal tissues was analysed using probes for human defensin 5 (HD5), lysozyme, secretory phospholipase A2 (sPLA2), and glyceraldehyde-3-phosphate dehydrogenase (G3PDH). Abbreviations for small intestine: D, duodenum; IC, ileocaecum; I, ileum; J, jejunum; Abbreviations for large intestine: A, ascending; D, descending; T, transverse; C, caecum; R, rectum. The autoradiographic exposure was three days with an intensifying screen at −80°C.