Figure 1.
Separation of pol IIIts from the UmuD′2C complex by using Superdex 200 gel filtration. (Upper) Superdex 200 fractions (30 μl) containing pol III α subunit [designated as α (Left)] were resolved on an 8% SDS/PAGE gel and visualized by chemiluminescent immunodetection by using antiserum directed against α subunit. (Lower) Superdex 200 fractions (20 μl) containing UmuC protein [designated as C (Left)] were visualized on a 12% SDS/PAGE gel stained with Coomassie blue R-250. The presence of UmuC and UmuD proteins was verified by using UmuC and UmuD′ antisera (data not shown).