Skip to main content
. 1999 Aug 3;96(16):8919–8924. doi: 10.1073/pnas.96.16.8919

Figure 2.

Figure 2

UmuD′2C (E. coli polV) has DNA polymerase activity. UmuD′2C was purified from a ΔpolB dnaE1026ts strain, RW588. Sephadex 200 fractions fx (50) containing predominantly pol IIIts; fx (56) containing pol IIIts + UmuD′2C and fx (64) containing UmuD′2C having no detectable pol IIIts were assayed for polymerase activity by extension of a 32P-labeled 30-mer primer annealed to a linear M13 DNA template, at permissive (37°C) and nonpermissive (47°C) temperatures. A single running-start base, C, is incorporated opposite G to reach the abasic lesion, X. The left-hand lane contains the primer (P) in the absence of proteins. Each reaction mixture contains 1.5 μl of the indicated fraction from a Superdex 200 gel filtration column (Fig. 1). All reactions were carried out in the presence of pol I antibody, RecA, SSB, β, γ complex, 4 dNTPs, and ATP.