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Kinetic analysis of the effect of wild-type pol III on UmuD′2C (E. coli pol V)-catalyzed translesion synthesis. Lineweaver–Burk double reciprocal plots for pol III HE and α HE (Upper) were generated from the kinetic data given in Fig. 4. Apparent Km and Vmax values and their ratios characterizing translesion synthesis by UmuD′2C (designated as Umu) in the absence and presence of pol III are provided in the tables below.
