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. 2007 Jan 12;401(Pt 3):701–709. doi: 10.1042/BJ20060990

Figure 6. Effect of mutations in the CTS of Cb5A on targeting to ER, mitochondria and liposomes.

Figure 6

Translation reactions containing Cb5AΔ/B, Cb5AΔ/mA, Cb5AΔ/R, Cb5AΔ/RR, Cb5AΔ/E, Cb5AΔ/EE or Cb5AΔ/Bcl were incubated in the presence or absence of ER membranes, mitochondria or liposomes as described in the legends of Figures 2 and 4. The percentage targeting reported is corrected for background by subtracting the percentage of protein in the bottom fraction in the absence of membrane from that in the presence of membranes. Targeting to mitochondria is shown by white bars, targeting to ER is shown by grey bars and targeting to liposomes is shown by black bars. Error bars represent standard deviation from the mean (n=3).