. 2007 Jan 12;401(Pt 3):679–688. doi: 10.1042/BJ20060555

Table 2. Kinetic and binding parameters of CENTG1 GLD and mutants.

Protein	Substrate	pH	V_max (μM·min⁻¹)	k_cat (min⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹·min⁻¹)
Wild-type	GTP	7.0	2.16	21.40	541	0.039
		8.0	0.43	4.62	442	0.010
		6.0	0.47	4.67	427	0.010
Wild-type	ATP	7.0	1.91	19.60	412	0.047
		8.0	0.45	4.89	378	0.013
		6.0	0.48	4.53	412	0.011
Wild-type	UTP	7.0	2.81	28.62	517	0.055
		8.0	0.65	6.73	467	0.014
		6.0	0.36	3.57	449	0.007
Wild-type	CTP	7.0	1.71	16.14	601	0.026
		8.0	0.33	3.06	516	0.006
		6.0	0.34	3.41	505	0.006
T101P	GTP	7.0	0.076	0.74	990
		8.0	0.012	0.12	889
		6.0	0.017	0.14	901
T101A	GTP	7.0	0.089	1.08	2681
		8.0	0.037	0.41	4026
		6.0	0.030	0.34	3062