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ICatSper was absent in CatSper−/− sperm. (a) Sperm (WT) whole-cell current evoked by a 1-s ramp from −100 to +100 mV, holding potential (HP) = 0 mV. The Na+-peak current recorded at −100 mV in DVF conditions was −970 pA and decreased to −13 pA in 2 mM Ca2+. (b) Monovalent current through CatSper channels (DVF solution) was absent in CatSper−/− sperm cells. (c) Average of the Na+ current in DVF solution measured from WT and CatSper−/− sperm cells.
