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. 2007 Jan 16;104(4):1219–1223. doi: 10.1073/pnas.0610286104

Fig. 4.

Fig. 4.

Interactions between CatSper proteins. (a) Epitope-tagged CatSper2 (FLAG-CS2), CatSper3 (HA-CS3), or CatSper4 (FLAG-CS4) were transfected into an MZ8 cell line stably expressing CatSper1 (CS1). FLAG-CatSper2, HA-CatSper3, and FLAG-CatSper4 were detected in the respective cell lysates (Left). After immunoprecipitation with anti-HA and -FLAG antibodies, immune complexes were probed with anti-CatSper1 antibody (Right). Negative controls were lysates from CatSper1 cells transfected with FLAG-GIRK4 or HA-TRPV6. (b) Proteins solubilized from testis microsomes were immunoprecipitated with specific anti-CatSper antibodies. Anti-CatSper3 and -CatSper4 pulled down CatSper3 and CatSper4 from WT testes but not from homozygous mutant testes (Left). These antibodies also pulled down their respective proteins from CatSper1−/− mice (Center). Anti-CatSper1 coimmunoprecipitated CatSper3 and CatSper4 in the same protein complexes from WT but not from CatSper1−/− testes (Right). Bottom Left shows CatSper1 in preparations used for immunoprecipitations; Bottom Right shows immunoprecipitation input control with anti-Na, K-ATPase, a plasma membrane protein.