Figure 5.
GA inhibits viral replication in poliovirus-infected animals without eliciting drug resistance. (A) Outline of the experiment. (B) Viral load in the brains of poliovirus-infected cPVR transgenic mice treated with vehicle or GA is expressed as number of PFUs per gram of brain (n = 10 per group, p < 0.01 by Wilcoxon two-sample test). (C) Viral populations recovered from GA-treated animals remain GA sensitive. Poliovirus isolated from the brains of infected animals from B was used to infect HeLa S3 cells at a low MOI (10−4) in the presence or absence of 1 μM GA. Virus production was measured after 48 h by standard plaque assay. Data represent the average number of PFUs produced per cell from all 10 GA-treated animals and four control animals.