Figure 2.

Effect of DCQ, IR and their combinations on cell cycle regulation in HCT116 cells exposed to oxic or hypoxic conditions. Cells were treated with 5 μM DCQ or DMSO (0.1%) and exposed to hypoxia or incubated in oxia for 1 hour, then irradiated (2 Gy). Immediately after radiation or drug treatment, cells were replenished with fresh medium containing no drug and incubated for another 24 hours. Cell cycle changes were assessed using Propidium iodide stain with flow cytometry as described in "Materials and Methods". Bar graphs are a summary of at least three independent experiments each performed in duplicates.