Skip to main content
. Author manuscript; available in PMC: 2007 Jan 16.
Published in final edited form as: Mol Endocrinol. 2006 Aug 24;20(12):3251–3262. doi: 10.1210/me.2006-0200

Fig. 1. CoCoA N-terminus has an activation function and serves as a coactivator for LEF1/β-catenin.

Fig. 1

A, 293T cells were transfected in 12-well plates with pGL3OT reporter plasmid (200 ng), pSG5.HA-LEF1 (1 ng), pSG5.HA-β-catenin (5 ng), and pSG5.HA-CoCoA or pSG5.HA-CoCoA (1-190) (200 or 400 ng) as indicated. Cell extracts were assayed for luciferase activity 48 h after transfection. B, STF cells were transfected in 24-well plates with pSG5.HA-β-catenin (2.5 ng), and pSG5.HA-CoCoA or pSG5.HA-CoCoA (1-190) (50 or 100 ng) as indicated. C, Structure of CoCoA N-terminus. LZ, leucine zipper motif. D, 293T cells were transfected in 24-well plates with GK1-Luc reporter plasmid (150 ng) and plasmids encoding Gal4-DBD or Gal4-DBD-CoCoA fusion proteins (100 ng) as indicated. Cell extracts were assayed for luciferase activity (right side) or analyzed by immunoblot with anti-Gal4 antibody (left side) 48 h after transfection.

HHS Vulnerability Disclosure