Fig. 3. p300 is specifically targeted to an integrated promoter by CoCoA N-terminus.
HLR cells in 150-mm dishes were transfected with Gal4-DBD or Gal4-DBD-CoCoA (1-149) (12 μg). 48 h after transfection, Chromatin immunoprecipitation assays were performed with the transfected cell extracts using the indicated antibodies. Quantitative PCR analysis of precipitated DNA was performed using primers that span the Gal4-responsive elements. Results shown are from a single experiment which is representative of three independent experiments.