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. Author manuscript; available in PMC: 2007 Jan 16.
Published in final edited form as: Mol Endocrinol. 2006 Aug 24;20(12):3251–3262. doi: 10.1210/me.2006-0200

Fig. 5. Synergistic enhancement of LEF1-mediated transcriptional activation by β-catenin, CoCoA, p300 and GRIP1.

Fig. 5

A, 293T cells were transfected in 24-well plates with pGL3OT reporter plasmid (100 ng), pSG5.HA-LEF1 (0.1 ng), pSG5.HA-β-catenin (1 ng), and a pSG5.HA vector encoding CoCoA full-length, CoCoA (1-500), or CoCoA (1-500)F17A (50 or 100 ng) as indicated. Cell extracts were assayed for luciferase activity 48 h after transfection. Inset, COS-7 cells in 12-well plates were transfected with CoCoA full-length, 1-500, or 1-500 F17A (500 ng). Cell extracts were analyzed by immunoblot with anti-HA antibody. B, STF cells were transfected in 24-well plates with pSG5.HA-β-catenin (2 ng), pCMV-p300 (1 ng), and pSG5.HA vectors encoding the indicated CoCoA species (60 ng) as indicated. Cell extracts were assayed for luciferase activity 48 h after transfection. Left inset, 293T cells were transfected with pGL3OT reporter plasmid (100 ng), pSG5.HA-LEF1 (0.1 ng), and the indicated quantities of pSG5.HA-β-catenin. Right inset, COS-7 cells in 12-well plates were transfected with plasmids encoding CoCoA full-length (FL) or the indicated CoCoA fragment or mutant (500 ng). Cell extracts were analyzed by immunoblot with anti-HA antibody. C, 293T cells were transfected in 24-well plates with pGL3OT reporter plasmid (100 ng), pSG5.HA-LEF1 (0.05 ng), pSG5.HA.β-catenin (0.5 ng), pSG5.HA-GRIP1 (50 ng), pCMV-p300 (50 ng), and pSG5.HA-CoCoA or pSG5.HA-CoCoA (1-500) (100 ng) as indicated. Cell extracts were assayed for luciferase activity 48 h after transfection.

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