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CO-based reduction of purified recombinant P450s by adrenodoxin/adrenodoxin reductase, rat P450 reductase, and E. coli Fld/Fdr
| Donor | SCC | bC17 | MT |
|---|---|---|---|
| Adx/Adr | 80% | — | NR |
| Rat P450 reductase | — | 73% | NR |
| Fld/Fdr | — | 77% | 20% |
Reduction is compared with 100%, determined by sodium hydrosulfite. Adx/Adr, adrenodoxin/adrenodoxin reductase; SCC, cholesterol side cleavage P450; bC17, bovine 17α-hydroxylase; MT, mycobacterial 14α-demethylase; NR, no reduction.
