Figure 1.

Different grades of standard microscopy video image of the endothelium. These very wide field images (750 000 μm²) were archived in TIFF format. (A) Adequate quality image with poorly delineated cell contours, mediocre contrast, large blocks of invisible cells. (B) Medium quality image with fairly well delineated cell contours but high background noise and large blocks of invisible cells. (C) Good quality image with well delineated cell contours, no background noise, cells visible on practically all the image except one fold zone (arrow). (D) Automated error detection tool. The contours of cells exceeding 1500 μm² or the form of which is unrealistic (longest length greater than double the shortest width) appear violet to alert the technician. He could then either validate the cells if he deemed them acceptable, or touch them up by adding or erasing certain segments. In this example, six cells were genuinely large in area (black arrows), the others needed touching up (red arrows). Scale bar 200 μm