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. 2002 Jul;86(7):801–808. doi: 10.1136/bjo.86.7.801

Table 1.

Mean endothelial cell densities (ECD) at the start and end of storage, from 17 French cornea banks using organ culture storage (for 1999, source: French storage centre register3)

Cornea bank Corneas delivered (number) Donor age (years) Death-procurement time (hours) ECD at start of organ culture (cells/mm2) Organ culture time (days) ECD at delivery (cells/mm2)
1 126 62.0 9.25 2140 15.4 1986
2 102 67.2 14.2 2441 15.5 2131
3 31 54.0 NA 2316 19 2190
4 288 71.5 29.4 nd 18.5 2218
5 85 58.0 14 NA 16 2300
6 211 66.0 NA 2390 16 2308
7 73 68.0 11 2660 18.5 2340
8 64 64.0 11 NA 16 2496
9 91 50.0 8 2680 16 2540
10 93 49.4 12.4 2748 14.5 2561
11 152 NA 12 2675 16 2567
12 267 61.0 8 2800 17.3 2600
13 107 70.7 13.4 2738 21 2643
14 49 64.0 9.75 2806 17.3 2710
15 133 67.5 11.7 2645 14 2745
16 142 64.4 8.3 3030 18 2848
17 411 NA NA NA NA NA

**Only bank 4 used a computer aided analysis tool based on digitisation by tracing with the mouse the perimeter of 100 cells visible on the screen, an objective but laborious and time consuming method. The ECDs of our bank (number 2) and of the 15 others were measured manually during this period. Since our bank has been using our prototype analyser, our mean ECDs have increased a mean 4% (data not presented) and now approach those of bank 4. ECD = endothelial cell density, NA = data not available, nd = analysis not done