Table 2.

Image quality.

Image quality	Score	Criteria
Good	2	Excellent view of cell contours
		Low background noise
		Cells visible on over ⅔ of image area
Medium	1	Good view of cell contours
		Moderate background noise
		Cells visible on ⅓ to ⅔ of image area
Adequate	0	Poor view of cell contours
		High background noise
		Cells visible on less than ⅓ of image area

For the analyses in tri-image mode, overall image quality was graded “good” if the three images obtained scores of 2/2/2 or 2/2/1, “medium” if the scores were 2/1/1, 2/1/0, or 1/1/1, and “adequate” if the scores were 1/1/0, 1/0/0, or 0/0/0