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. 1999 Aug 3;96(16):8943–8948. doi: 10.1073/pnas.96.16.8943

Figure 1.

Figure 1

Identification of a <20-nt poly(A) tail on transferrin pre-mRNA. Poly(A) tail lengths on transferrin mRNA and pre-mRNA were determined by RT-PCR poly(A) analysis of a preparation of total Xenopus liver RNA. RT was primed with an oligo(dT) primer-adapter as described (21), and poly(A) tail lengths on transferrin mRNA and pre-mRNA were determined by PCR amplification using 5′ 32P-labeled primers for the terminal exon (lane 3) or the terminal intron (lane 4), respectively. For comparison poly(A) length was determined on albumin mRNA (lane 2) and pre-mRNA (lane 5) (21). The products of the PCRs were visualized by autoradiography after electrophoresis on 6% polyacrylamide/urea gels. M, molecular size marker HinfI-digested OX174 DNA.